圈门策略：
小鼠心脏细胞流式分选或分析需根据细胞类型、实验目的选择合适的圈门策略。以下是关键要点和方案。
巨噬细胞圈门策略：
?表面标志物选择?：CD11b（单核/巨噬细胞标志）结合F4/80（巨噬细胞特异性抗原）可有效区分巨噬细胞群体。 ?
?散射参数?：前向散射（FSC）和侧向散射（SSC）可辅助筛选细胞大小和复杂度，排除杂质细胞。 ?
?补偿调节?：需对FITC、PE等荧光染料进行补偿设置，避免信号溢漏导致假阳性。 ?
论文信息：
论文题目：Cardiac macrophages prevent sudden death during heart stress
期刊名称：Nature Communications
时间期卷：12, Article number: 1910 (2021)
在线时间：2021年3月26日
DOI：doi.org/10.1038/s41467-021-22178-0
产品信息：
货号：CP-005-005
规格：5ml+5ml
品牌：Liposoma
产地：荷兰
名称：Clodronate Liposomes& Control liposomes
办事处：Target Technology(靶点科技)
Clodronate Liposomes氯膦酸盐脂质体清除肝脏和肿瘤巨噬细胞，疾病模型为：肺动脉环缩术(pulmonary artery banding, PAB)模型。Pulmonary artery banding (PAB) 是通过环束带限制肺动脉血流的姑息性手术，主要用于治疗先天性心脏病，如室间隔缺损、大动脉转位等伴随过度肺血流的疾病。 ?原理: 该手术通过聚四氟乙烯带环束肺动脉主干，降低肺动脉压力和血流量，缓解心脏负担。早期作为复杂先心病分期治疗的过渡手段，现仅用于多发性室间隔缺损、合并主动脉缩窄等特殊病例。荷兰Liposoma巨噬细胞清除剂Clodronate Liposomes见刊于Nature Communications：心脏巨噬细胞在心脏压力期间防止猝死。
Liposoma巨噬细胞清除剂Clodronate Liposomes氯膦酸二钠脂质体的材料和方法文字描述：
Male C57BL6/J mice were purchased from CLEA Japan and Jackson Laboratory and maintained on a standard mouse chow diet under sterile barrier conditions, on a 12-h light-dark cycle with 18–23?°C and 40–60% humidity. Male Areg homozygous null (Areg?/?) mice were backcrossed to C57BL6/J mice over ten generations. Rag2?/?, Cd4?/?, and Cd8a?/? mice were purchased from Taconic (Germantown, NY). The genotypes of all mice were determined using genomic PCR. The isoproterenol challenge entailed bolus administration of isoproterenol (Sigma) 5?mg/kg intraperitoneally under constant ECG recording. Clodronate liposomes and control liposomes were purchased from LIPOSOMA B.V. A total of 10?μl of clodronate or control liposome solution per gram of mouse were intravenously administrated 24?h before PAB. After the first administration of clodronate or control liposomes, the same volume of liposomes was also administrated every 7 days for long term depletion, per the manufacturer’s instructions. All protocols for animal experiments were approved by the Animal Care and Use Committee of the University of Tokyo.
Liposoma巨噬细胞清除剂Clodronate Liposomes氯膦酸二钠脂质体的材料和方法文献截图：
